Abstract
Vinca alkaloids are a subset of drugs obtained from the periwinkle plant they are naturally extracted from the pink periwinkle plant (Catharanthus roseus), and they have been used to treat diabetes, high blood pressure and have been used as disinfectant. The vinca alkaloids are also important for being Cancer resistant there are four major vinca alkaloids in clinical use: Vinblastine (VBL), vinorelbine (VRL), vincristine (VCR) and vindesine (VDS). Vinca alkaloids are the second most used class of cancer drugs and will stay among the original cancer therapies. Catharanthus roseus is still source used for the powerful antitumor drugs. Callus culture had been done on MS-medium containing Different Concentrations from BA – 2,4-D – NAA – IAA. Where the different effects of growth regulators were studied when different parts of the plant have been cultured from leaves and stems this is to induce callus formation and encourage growth and the concentrations that were prepared: BA (0,1 – 0,3 – 1 – 2 mg/l) - NAA (0,1 – 1 mg/l) - IAA (0,1 mg/l) - 2,4-D (0,75 – 1 mg/l). And after culture the explants we transferred it to the growth room with a temperature ranging from 27 to 29 ° C relative humidity around 80% And in complete darkness. The results from HPLC analysis for the dry weight of callus were as follows ajmalicine (0.18 mg/g DW), catharanthine (0.07 mg/g DW), serpentine (0.37mg/g DW), vindoline (0.11 mg/g DW).
Keywords
References
- George, E. F., Hall, M. A., & Klerk, G. J. De. (2008). The Components of plant tissue culture media I: Macro- and micro-nutrients. PlantGoogle Scholar ↗
- Propagation by Tissue Culture 3rd Edition, 1, 65–113. https://doi.org/10.1007/978-1-4020-5005-3_3DOI ↗Google Scholar ↗
- Ghareeb, Y., Soliman, S., Ismail, T., & Hassan, M. (2016). EFFECT OF GROWTH REGULATORS AND ECOTYPES OF EGYPTIAN VINCA ROSAGoogle Scholar ↗
- (Catharanthus roseus) ON CALLUS INDUCTION AND ALKALOIDS PRODUCTION. Zagazig Journal of Agricultural Research, 43(1), 141–150.Google Scholar ↗
- https://doi.org/10.21608/zjar.2016.101575DOI ↗Google Scholar ↗
- Hoda, E. (2013). In Vitro Regeneration and Somaclonal Variation of Catharanthus roseus Don. Using Leaf and Internodal Explants.Google Scholar ↗
- Alexandria Science Exchange Journal: An International Quarterly Journal of Science Agricultural Environments, 34(October- December),Google Scholar ↗
- –459. https://doi.org/10.21608/asejaiqjsae.2013.3108DOI ↗Google Scholar ↗
- Ibrahim, M., Ahmed, M. F., Kazim, S. M., Ghori, S. S., Mehjabeen, S. S., Ahmed, S. R., & Ali, S. M. (2010). Antidiabetic activity of vinca roseaGoogle Scholar ↗
- extracts in alloxan-induced diabetic rats. International Journal of Endocrinology, 2010. https://doi.org/10.1155/2010/841090DOI ↗Google Scholar ↗
- Swanberg, A., & Dai, W. (2008). Plant regeneration of periwinkle (Catharanthus roseus) via organogenesis. HortScience, 43(3), 832–836.Google Scholar ↗
- https://doi.org/10.21273/hortsci.43.3.832DOI ↗Google Scholar ↗
- Ataei-Azimi, A., Delnavaz, H. B., Ebrahimzadeh, H., & Majd, A. (2008). High in vitro production of ant-canceric indole alkaloids fromGoogle Scholar ↗
- periwinkle (Catharanthus roseus) tissue culture. African Journal of Biotechnology, 7(16), 2834–2839.Google Scholar ↗
- https://doi.org/10.4314/ajb.v7i16.59172DOI ↗Google Scholar ↗