https://everant.in/index.php/jlsb <p>Welcome to the Journal of Life Science and Biotechnology, a prestigious platform dedicated to advancing research and knowledge in the vibrant fields of life sciences and biotechnology. Our journal serves as a focal point for scientists, researchers, educators, and industry professionals to share their discoveries, exchange insights, and contribute to the ongoing progress of these dynamic disciplines. With a commitment to scientific rigor and innovation, we aim to cultivate an intellectual community that propels the frontiers of life sciences and biotechnology.</p> Everant Publisher Pvt Ltd en-US Journal of Life Science and Biotechnology 2456-1061 https://everant.in/index.php/jlsb/article/view/559 <p>Vocabulary and successful reading comprehension appear to be intertwined, according to top researchers in the field. This paper will explore research about the latest methods that are proven to work in vocabulary instruction and how to effectively implement them in the classroom. Experts in the field will be identified and their findings explored in this crucial area of literacy. Strategies and techniques along with ways to integrate technology to enhance learning will be provided to give a complete vision of the process of teaching vocabulary, and thus improve reading comprehension.</p> Jerilou J. Moore, Ph.D. Copyright (c) 2023 2023-01-01 2023-01-01 148 https://everant.in/index.php/jlsb/article/view/560 <p>Diabetes is the fifth leading cause of death in most developed countries. In spite of this, the drugs available in the market for treatment of diabetes are more expensive with side effect. Therefore, there is a high demand of cost-effective novel natural antidiabetic drug without any side effect. In our investigation, we studied <em>in silico</em> the mechanism of secondary metabolites isolated from <em>S. persica</em> which modify the enzyme action, their <em>in vitro</em>&nbsp; &nbsp;inhibitory activity of alpha amylase and &nbsp;the phyto-constituent analysis those are responsible for its activity. <em>In silico</em> study revealed that kaempferol from <em>S. persica</em> is a most effective metabolite which can bind, interact and modulate the activity of all enzymes with much higher binding affinity and lesser binding energy such as -4.58 kcal/mol for alpha amylase, -4.76 kcal/mol for beta glucosidase, -4.97 kcal/mol for glycogen synthase kinase, -4.3kcal/mol for glucokinase and -3.85 kcal/mol for alpha glucosidase. Aqueous extract of <em>S. persica</em> has a high ability with 72.39% inhibition value to inhibit the activity of alpha amylase and 376μg /ml IC₅₀ value in comparison with standard drug which show 65.99% inhibition value. Total 15 compounds have been detected in TLC analysis in different solvent system and different extracts. Kaempferol might be one of them, which attribute to the anti-diabetic property. Further purification and characterization of kaempferol is required, which may prove a probable anti-diabetic drug</p> Twinkle S. Bansode Copyright (c) 2023 2023-01-01 2023-01-01 149 158